张可 牟玲
（日照市人民医院，山东 日照，276800）
中图分类号：R285.5 文献标识码：A 文章编号：1674-7860（2016）18-0031-02
【摘 要】目的：探讨海藻硫酸多糖的分离纯化及其抗肿瘤作用。方法：选取鼠龄、质量大致相同的昆明种雄性小鼠60只，随机分为5组，正常对照组、阳性对照组、低剂量组、中剂量组和高剂量组，每组12只。低剂量组、中剂量组和高剂量组分别注射浓度为25mg/ kg、50 mg/ kg、100 mg/ kg的海藻硫酸多糖，氟脲嘧啶20mg/kg注射阳性对照组，正常对照组采用生理盐水溶液注射，比较各组小鼠的抑瘤率。结果：低剂量组、中剂量组、高剂量组和阳性对照组小鼠的抑瘤率分别为5.0%、22.3%、27.9%和50.3%，阳性对照组的抑瘤率明显高于其他三组，高剂量组的抑瘤率明显高于低剂量组和中剂量组，中剂量组的抑瘤率明显高于低剂量组，组间比较差异具有统计学意义（P＜0.05）。结论：海藻硫酸多糖具有显著的抗肿瘤作用。
【关键词】海藻硫酸多糖；分离纯化；抗肿瘤作用
【Abstract】Objective:To explore the separation and purification of algae sulfated polysaccharide andits anti-tumor effect. Methods:60 Kunming male mice ofsimilar age and quality were selected and randomly divided into 5 groups: thenormal control group, the positive control group, low dose group, middle dosegroup and high dose group, with 12 rats in each group. Low-dose group, middledose group and high dose group were injected at a concentration of 25mg / kg,50mg / kg, 100mg / kg of seaweed sulfated polysaccharide respectively, thepositive control group was given fluorouracil 20 mg / kg injection while the normalcontrol group was injected with saline solution, inhibitory rate of each groupof mice was compared. Results: The inhibition rate of low-dose group, middledose group, high dose group and the positive control group was 5.01%, 22.3%,27.9% and 50.3% respectively, the inhibition rate of the positive control groupwas significantly higher than that of the other three groups, the inhibitionrate of high-dose group was significantly higher than that of the low dosegroup and middle dose group, the inhibition rate of middle dose wassignificantly higher than the low-dose group, the difference between the groupswas statistically significant (P<0.05). Conclusion: Seaweed sulfated polysaccharides had significantanti-tumor effect.
【Keywords】Seaweedsulfated polysaccharides; Isolation and purification; Antitumor effect
doi:10.3969/j.issn.1674-7860.2016.18.013